ICSI (Intracytoplasmic Sperm Injection), is a procedure where a single sperm is injected into the egg. To achieve fertilization the sperm is withdrawn into a tiny pipette, the egg fixed with a holding pipette and the sperm injected into the cytoplasm of the metaphase II (mature Oocyte). After the ICSI procedure the Oocytes are incubated and the resultant embryos are transferred into Uterine cavity by using a specially designed embryo transfer catheter. After the embryo transfer, Progesterone supplementation is given. The confirmation of Pregnancy done with estimation of HCG and after 6 weeks for the foetal heart on ultrasound.
With ICSI procedure, men with severe oligo asthenospermia and azoospermia have an opportunity to produce their own biological child, without resorting to Donor Insemination or Adoption. In cases of Congenital Bilateral Vasal Agenesis (absence of VAS), failed vasoepididymal anastomosis(VEA) and non obstructive azoospermia, ICSI has proved to be successful. In cases of repeated failure with conventional In Vitro fertilization, ICSI could be considered to maximize the chances.